Table 3.

IRSp53 and its –SH3 domain interactor proteins (Mena, Eps8 and Dynamin) localisation within filopodia.

Protein Localisation	IRSp53	Mena	Eps8	Dynamin
Tip	+	+++	++	−
Shaft	−	+	+	−
Base	+	−	−	+++
Membrane	++	−	−	−

HA-IRSp53 was coexpressed with either GFP fusions of Mena, Eps8 or Dynamin in duplicate experiments on N1E-115 cells and labelled for respective proteins using anti-GFP Alexa 647 conjugate in one set of experiment. In the other set HA-IRSp53 was labelled with anti-HA-Alexa647. The dSTORM images were obtained as described in experimental methods. The observed protein localisation within filopodia was compared visually and presented. Representing (−) no localisation, (+) being lowest and (+++) highest localisation (n = 5 per protein).