Fig. 5.

Transcriptome comparison. Transcriptomes (RNA-seq) of B. subtilis ∆spoIIE were analyzed for cells incubated 14 days in starvation buffer (in triplicate), and for cells grown in Spizizen minimal medium (SMM) and harvested in exponential or stationary growth phase, respectively (each in duplicate). a Principal component analysis plot of the different samples. Samples were plotted against the first two principal components calculated from the gene expression values. The axes labels indicate percentage of total variance that is explained by each component. b Heat map of the averaged transcriptome profiles. Expression levels were transformed to Z-scores. Green indicates low expression (Z-score = −3.5), yellow indicates average expression (Z-score = 0), and red indicates high expression (Z-score = +3). c Venn diagram indicating the number of upregulated and downregulated genes in 14-day-starved cells compared to exponential growth phase cells (left) and stationary growth phase cells (right). Strain used: B. subtilis PG344 (spoIIE::erm)