Fig. 2. Amot-p130 inhibits the EMT of breast cancer cells.

a A photomicrograph of a scratch created with a 200 μl pipette tip in the wound-healing assay at 0 and 24 h. b Cell migration and c cell invasion were assessed using transwell assay (left). The number of migrated or invaded cells was expressed as the mean ± SD of three independent experiments (right). d Expression of epithelial to mesenchymal transition (EMT) markers was determined by western blotting. β-actin was the loading control. e The subcellular localization of EMT markers was determined by immunofluorescence staining. Both E-cadherin and vimentin antibodies were diluted at a ratio of 1:200. DAPI was used for nuclear staining (blue). Scale bars = 25 µm. All P values were calculated by Student’s t test. *P < 0.05, **P < 0.01. DAPI, 4′,6-diamidino-2-phenylin