Fig. 4. Amot-p130 inhibits the CSC potential of breast cancer cells.

a ALDH-positive population was detected by flow cytometry (left). The proportions were calculated from three independent experiments (right). b CD24-low/CD44-high populations were detected by flow cytometry (left). The proportions were calculated from three independent experiments (right). c The tolerance of MCF7 cells to tamoxifen and MM231 cells to cisplatin was determined by MTT assay. d Stemness markers were assessed by western blotting. GAPDH was used as the loading control. e The formation of tumor spheres was recorded under microscopy (left). Scale bar = 50 µm. The proportions were calculated from three independent experiments (right). f Xenograft formation in NOD-SCID mice evaluated at 6 weeks after injection. Tumor volumes were determined using the formula volume = 1/2 × length × width². g The number of outgrowths was recorded at the end of the 6th week using limited-dilution assays. h IHC analysis of protein expression of Amot-p130, E-cadherin, and vimentin in xenograft tissues. Scale bar = 200 µm. All P values were calculated by paired Student’s t test. *P < 0.05, **P < 0.01. IHC, immunohistochemistry; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide