Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 21;10:891. doi: 10.1038/s41467-019-08869-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — PglS can glycosylate the acceptor protein ComP with the pneumococcal CPS14 polysaccharide. Escherichia coli SDB1 cells co-expressing an acceptor protein (DsbA, AcrA, or ComP), an OTase (PglL, PglB, or PglS), and the CPS14 polysaccharide were analyzed for protein glycosylation via western blot analysis of the affinity-purified acceptor proteins. a–c DsbA purified from SDB1 cells in the presence or absence of PglL. a Anti-His channel probing for Hexa-histidine tagged DsbA. b Anti-glycan channel probing for CPS14. c Merged images for panels a and b. d–f AcrA purified from SDB1 cells in the presence or absence of PglB. d Anti-His channel probing for Hexa-histidine tagged AcrA. e Anti-glycan channel probing for CPS14. f Merged images for panels d and e. g–i ComP purified from SDB1 cells in the presence or absence of PglS. g Anti-His channel probing for Hexa-histidine-tagged ComP. h Anti-glycan channel probing for CPS14. i Merged images for panels g and h. The red asterisk indicates samples that were proteinase K treated for 1 h at 55 °C. Source data are provided as a Source Data file