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. 2019 Feb 21;10:891. doi: 10.1038/s41467-019-08869-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Immunoglobulin G (IgG) responses of mice vaccinated with ComP, Prevnar 13^®, a monovalent bioconjugate and a trivalent bioconjugate. Groups of mice were vaccinated with ComP alone, Prevnar 13^®, a monovalent CPS14-ComP bioconjugate vaccine, or a CPS8-/CPS9V-/CPS14-ComP biconjugate vaccine. Sera were collected on day 49 and analyzed for serotype-specific IgG responses via enzyme-linked immunosorbent assay (ELISA) compared against sera collected on day 0. a–c No IgG responses were detected in placebo vaccinated-mice for serotypes 8 (a), 9V (b), or 14 (c). d–f Prevnar 13^®-vaccinated mice did not have detectable IgG responses to serotype 8 (d), but did have IgG responses specific to serotype 9V (e) and 14 (f). g–i Mice vaccinated with a CPS14-ComP bioconjugate vaccine did not have IgG responses to serotypes 8 (g) or 9V (h), but did have IgG responses to serotype 14 (i). j–l Trivalent CPS8-/CPS9V-/CPS14-ComP bioconjugate vaccinated mice all had statistically significant IgG responses to serotypes 8 (j), 9V (k), and 14 (l). Unpaired t tests (Mann–Whitney) were performed to statistically analyze pre-immune sera from day 49 sera. P values for each case tested were ****p = 0.0001. Each dot represents a single vaccinated mouse (n = 10 mice per group). ELISA statistical calculations were performed on sera samples run in technical triplicates. Error bars indicate the standard deviation of the mean. Source data are provided as a Source Data file