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. 2019 Feb 21;9:2456. doi: 10.1038/s41598-018-38349-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Global approach using whole image saturation and determination of the influence of the acquisition time on reconstruction pixel size. (A) Saturated Images for different pixel sizes and after different acquisition times. (B) Corresponding saturating curves with the tangent at origin intersecting the 100% saturation line to determine the saturation time. (C) Experimental time constants as a function of the pixel size and inverse fit $y = p_{1} \frac{1}{x}$ with p₁=384 s. μm⁻¹, R² = 0.99.