Fig. 1.

Claspin, Timeless, and CHK1 mRNA are overexpressed in primary colorectal, lung, and breast cancers. a Schematic representation of the ATR pathway showing the six checkpoint factors analyzed in this study. b Relative mRNA levels of ATR, RAD9, RAD17, Claspin, Timeless, CHK1, and PCNA in 93 lung tumors, 74 primary colorectal carcinomas, and 206 breast cancer samples. mRNA levels were determined by quantitative RT-PCR and are expressed as the ratio of tumor (T) to normal tissues (N). c Nonparametric Spearman correlation analysis of the expression of checkpoint genes and proliferation marker (PCNA) in lung, colorectal, and breast cancer samples. d High expression of Claspin, Timeless, and CHK1 is associated to bad prognosis in NSCLC patients. Disease-free survival (DFS) according to gene level expression: Claspin, Timeless, CHK1, RAD17, ATR, and RAD9. Totally, 72 patients were analyzed