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. 2019 Feb 21;9:2436. doi: 10.1038/s41598-019-38857-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of (A) wild-type or (B) PT6 prothrombin to FVa using surface plasmon resonance. Wild-type (A) or PT6 (B) prothrombin at varying concentrations (0, 78, 156, 313, 625, 1250, 2500, 5000 and 10,000 nM) were injected into a flow cell containing immobilized FVa for 60 s prior to washing with HBSC Tween-80. K_d values were determined using a kinetic 1:1 model.