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. 2019 Feb 21;9:2529. doi: 10.1038/s41598-019-39844-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blotting of HEK293 cells expressing various truncated spike proteins. Cell lysates of HEK293 cells expressing different lengths of S protein were separated by SDS-PAGE, transferred to PVDF membranes, probed with an anti-V5 tag antibody, and detected using HRP-conjugated secondary goat anti-mouse immunoglobulin antibody. The protein ladder/marker is shown in kilodalton (kDa). The bands corresponding to the various truncations of the spike protein are (a) a.a. 1–435; (b) a.a. 1–485; (c) a.a. 1–501; (d) a.a. 1–509; (e) a.a. 1–575; (f) a.a. 1–639; (g) full-length spike protein.