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. 2019 Feb 21;9:2529. doi: 10.1038/s41598-019-39844-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mapping epitopes by ELISA targeting different truncated PEDV S proteins. The six truncated as well as full-length spike protein of PEDV-PT were coated into separate wells and probed with two-fold serially diluted mAbs, P4B-1 and E10E-1–10. The non-PEDV recognizing mAb, UNK-1, was added as the external control for ELISA under the same dilution conditions. The two-fold serially diluted anti-V5 tag antibody was used as the standard to show the dilution effect of the binding affinity assay. The data is represented as the S/P ratio. The wells incubated with only secondary antibodies and the wells incubated with anti-V5 tag antibody at 1,250 ng/mL were used as negative and positive controls for S/P ratio respectively. The results of the ELISA for the various truncations of the spike proteins are shown as follows: (a) S^1-435; (b) S^1-485; (c) S^1-501; (d) S^1-509; (e) S^1-575; (f) S^1-639 and (g) full-length S.