Table 2.
Thermodynamic binding parameters of RiMnBP to linear and decorated manno-oligosaccharides
| Ligand |
K
d
(µM) |
∆G (kcal mol−1) |
∆H (kcal mol−1) |
−T∆S (kcal mol−1) |
n |
|---|---|---|---|---|---|
| M3 | 2.62 | −7.6 | −33.2 | 25.6 | 0.7 |
| M4 | 3.89 | −7.4 | −28.6 | 21.2 | 0.7 |
| M5 | 2.55 | −7.7 | −21.8 | 14.1 | 0.8 |
| M6 | 33.75 | −6.2 | −17.8 | 11.6 | 0.5 |
| M4Ac2 | 25.65 | −6.3 | −21.9 | 15.6 | 0.9 |
| M5Ac2 | 23.53 | −6.3 | −20.2 | 13.9 | 0.8 |
Binding was measured by ITC. Data are means of two independent titrations
Kd dissociation constant, ΔG Gibbs free energy, ΔH enthalpy, −TΔS entropy, n binding stoichiometry