Fig. 4.

SsD delays the endocytosis pathway in HeLa cells and does not alter Ca²⁺ content in the lysosome or ER. a HeLa cells were treated with EGF in the presence or absence of SsD (15 μM) and subjected to EGFR immunoblot analyses. b SsD (15 μM) pretreatment for 6 h failed to affect the ability of TG (1 µM) to release ER Ca²⁺ in Fura-2-loaded HeLa cells in the absence of extracellular Ca²⁺. c SsD (15 μM) pretreatment for 6 h did not affect the ability of GPN (200 µM) to release lysosomal Ca²⁺ in Fura-2-loaded HeLa cells in the absence of extracellular Ca²⁺. The graphs in (b) and (c) represent data from three independent experiments, and data are expressed as the means ± S.D., n = 3. The * symbols indicate p < 0.05 by t-test analysis