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. 2019 Feb 22;10(3):183. doi: 10.1038/s41419-019-1432-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The expression levels of the indicated miRNAs in embryonic stem (ES) cells and various mouse tissues were plotted using the datasets in GEO. b The expression levels of the indicated miRNAs during C2C12 cells differentiation were analysed by RT-qPCR (top panel) or by using the datasets in GEO (bottom panel). c–g C2C12 cells were transfected with the indicated miRNA mimics, and induced differentiation for 4 days. c The mRNA levels of SETD3 and CCNE1 from differentiated cells were examined by RT-qPCR. d The differentiation protein markers in the transfected cells were analyzed by western blot analysis. e Immunofluorescence staining was performed using a specific antibody against MHC (red). DAPI stains nuclei. Scale bar: 100 μm. f MHC-positive cell numbers in certain field of the indicated C2C12 cells describe in e were quantified. g Quantitative analyses of nuclei number per fiber from the indicated C2C12 cells. Data represent the mean ± SD from three independent experiments