Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 21;2:74. doi: 10.1038/s42003-019-0317-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — SuperSIL resolution characterization and imaging of E. coli cells under cryogenic conditions. Representative images of sub-diffraction limited objects from a the superSIL microscope and b the specialist system. Scale bar: 0.5 µm. c Box charts of FWHM of the PSFs for the superSIL and specialist systems showing the 25th and 75th percentiles of the data points. The thick line with a square at the center shows the mean value; the thin line in the box shows 50th percentile. The mean of FWHM was 153 ± 14 and 328 ± 86 nm in the case of superSIL and 0.9 NA objective, respectively. The whiskers show the standard deviation. d ATP-binding cassette (ABC) transporter protein PH1735 fused with EGFP in E. coli cells imaged in wide-field superSIL microscopy and e superSIL STORM. f Localization precision histogram from the image in e. g The enlarged image of the region of the cell indicated by the red dashed border box in e. h The enlarged image of the small region in the cell indicated by the orange dashed border box in g. i Line profile of the cross-section of two PH1735 protein clusters indicated by the cyan dashed lines in h. j Line profile of the cross-section of two PH1735 protein clusters closer to each other indicated by the red dashed line in h. k Line profiles of the cross-section of two adjacent single molecules (magenta and green) indicated by the magenta and green dashed lines in h. l FRC curve revealing the 12 nm resolution in the region shown in h. Scale bars: 1 μm (d, e), 100 nm (g) and 20 nm (h)