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. 2019 Feb 21;2:74. doi: 10.1038/s42003-019-0317-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — SuperSIL resolution performance at room temperature. a Image of a point object (top). Scale bar: 0.5 µm. The box chart (bottom) of the full width half maximum (FWHM) measurements of images from point emitters. The boxes show the 25th and 75th percentiles of the data points. The thick line with a square at the center shows the mean value; the thin line in the box shows 50th percentile. The mean of FWHM was 153 ± 15 nm. The whiskers show the standard deviation. b Comparison of wide-field images of live McjD-EGFP in E. coli cells. The superSIL image (left) has ×471 magnification, while the specialist microscope (right) has ×100 magnification. For comparison the inset contains a ×4.71 scaled-up image of a cell. Scale bar: 5 µm, and 1 µm in the inset. c STORM images (top) and localization precision histograms (bottom) of live McjD-EGFP E. coli cells in superSIL STORM (left) and specialist STORM (right). The insets show wide-field fluorescence images of the cells. Scale bar: 1 µm. d Resolution evaluation of STORM imaging in the specialist microscope. Left: Schematic of DNA origami nanorulers labelled with ATTO 647N dye molecules. Right: image of a field of nanorulers obtained from wide-field (top left) and STORM (top right). Scale bar: 200 nm. The STORM image of a nanoruler is shown in the inset, indicated by the white-border box. Scale bar: 20 nm. Bottom left: Localization precision histogram from the STORM image of the nanorulers. Bottom right: Profile of the cross-section of the nanoruler (magenta dots) in the magnified inset, and its Gaussian fit (magenta line)