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. 2019 Feb 21;9:2520. doi: 10.1038/s41598-018-37525-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Coupling patterns at the C2/H2 resonances of serine and creatine in [U-¹³C,¹⁵N]serine labelled U266 cells from ¹H-¹³C-HSQCs. The serine C2 resonance is predominantly a doublet of doublets of doublets because of J_C1C2 coupling (53.1 Hz), J_C2C3 coupling (36.9 Hz) and J_C2N2 coupling (5.9 Hz). The C2 resonance of creatine is a doublet of doublets as the glycine-derived moiety gives rise to a J_C1C2 coupling (54.5 Hz) and J_C2N2 coupling (11.8 Hz).