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. 2019 Feb 22;9:2584. doi: 10.1038/s41598-019-39201-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Abnormalities in development in the plants grown at 6, 21 and 24 °C. At the lowest temperatures plants headed but anthers were not extruded, at 21 °C more than half of the plants of NILs with early alleles headed (left image) while none of the plants reached heading in the NILs with the late allele (right image) and at 24 °C none of the plants progressed in their development to heading. Pictures were taken at the end of the experiment.