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. 2019 Feb 22;9:2584. doi: 10.1038/s41598-019-39201-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relationship between the rate of development between the onset of the experiment and heading and temperature for lines carrying Eps-early (left panel) or Eps-late (right panel) alleles grown under constant temperatures of 6, 9, 12, 15, 18, 21 and 24 °C. To avoid a biased estimate of parameters, lines were fitted by bi-linear regression fitted with data at temperatures between the base and the maximum thresholds (for lines carrying Eps-early 6–24 °C and for Eps-late 6–21 °C). Insets are the calculated cardinal temperatures (base, T_base; optimum, T_opt; and maximum, T_max) for each group of NILs.