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. 2019 Feb 22;9:2584. doi: 10.1038/s41598-019-39201-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thermal time from the onset of the experiment to heading (estimated using 0 °C as base temperature) for lines carrying Eps- early (hatched bars) or Eps-late (solid bars) alleles grown under constant temperatures of 6, 9, 12, 15, 18, and 21 °C. Inset is a detail of the difference (in °C d) between lines with the Eps-late or Eps-early alleles. Asterisks indicate the statistical significance of the differences between lines with late and early alleles at each temperature from the LSmeans contrast (**P < 0.01, ***P < 0.001).