Fig. 7. A-1210477-induced apoptosis does not involve mitochondrial dysfunction.

a Jurkat wt cells were incubated with 0, 3, or 20 µM A-1210477 for 6 h or 50 µM CCCP for 5 min as indicated then stained with 2.5 µM JC-1 for 15–30 min. Additionally, cells were incubated concurrently with 20 µM QVD. Each sample was analyzed by flow cytometry to detect JC1 monomers and aggregates. b NB4 cells were incubated with 0, 1, 5, or 10 µM cyclosporine A alone or with 20 µM A-1210477 and analyzed for PARP cleavage. The pan-caspase inhibitor, QVD, was used as a positive control