Fig. 3.

Type I + II IFN signaling do not explain the defective expansion of Stat1^−/− T cells. Il10rb^−/−Rag1^−/− mice were injected i.p. with 1 × 10⁶ WT, Ifnar1^−/−Ifngr1^−/− or Stat1^−/− CD4⁺ T cells. a Representative flow cytometry plots of CD4⁺ T cells (gated on live CD45⁺ cells, Supplementary Fig. 4b) in the spleen and colon followed by their mean frequencies ± SEM at 3 weeks post transfer. b Mean % initial body weights ± SEM following T-cell transfer. Source data are provided as a Source Data file. c Representative images of colons, as well as representative H&E images of distal colon sections with mean histological scores ± SEM at 3 weeks post transfer. Scale bar represents 200 µm. d Representative flow cytometry plots of CD4⁺ T cells (gated on CD45⁺ cells) in the blood followed by their mean frequencies ± SEM at 2 and 3 weeks post transfer. All data are pooled from two to three independent experiments, with each point representing an individual mouse. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by b two-way ANOVA with Bonferroni’s correction (WT compared to Ifnar1^−/− Ifngr1^−/− or Stat1^−/−, Ifnar1^−/− Ifngr1^−/− compared to Stat1^−/−) or by a, c, d two-tailed Mann–Whitney test