Fig. 3. Kv11.1 stimulation inhibits reprograms EMT.

a Representative western blot images of EMT markers (N-cadherin, Vimentin, CD44, and E-cadherin) in MDA-MB-231, SKBR3, and MCF7 breast cancer cells treated with either vehicle control or NS1643. Cells were treated with 50 μm of NS1643 for 24 h, harvested and subjected to western blot analysis. Actin was used as a loading control. Quantification of the levels of Vimentin, CD44, N-Cadherin, and E-cadherin was done using NIH ImageJ software. Data are expressed as mean ± SEM; *p < 0.005; **p < 0.001; n = 3). b Representative western blot images of EMT markers, Vimentin and CD44, in MDA-MB-231-derived tumors from mice treated with either vehicle control or NS1643. c The mRNA levels of Snail, Slug, Twist, and Zeb were represented relative to β-actin transcripts. Cells were treated with 50 μm of NS1643 for 24 h, harvested, and subjected to qRT-PCR analysis. Data are expressed as mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.00005