Fig. 4. Kv11.1 stimulation suppresses the stemness of breast cancer cells.

a Representative images are showing the effect of Kv11.1 stimulation on mammosphere forming efficiency (MFE) in SKBR3 and MCF7. Cells were seeded into Ultra-low attachment 6-well plate under DMSO or 50 μm of NS1643 for 7 days. b The mRNA levels of Oct4, Nanog, and Sox were represented relative to β-actin transcripts. Cells were treated with 50 μm of NS1643 for 24 h, harvested, and subjected to qRT-PCR analysis. Data are expressed as mean ± SD of three independent experiments. *p < 0.01; **p < 0.05; ***p < 0.0005