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. 2019 Jan 9;98(3):313–321. doi: 10.1177/0022034518818449

Figure 2.

Figure 2.

AdipoRon (APR) promotes osteogenesis and mineralization in vitro. Calvarial bone osteoblasts were isolated and induced to undergo osteogenesis. Alkaline phosphatase (ALP) staining (A) and Alizarin red (AR) staining (B) of osteogenesis induced calvarial bone osteoblasts treated with or without APR at 80 µM (scale bar = 100 µm). (C) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) of ALP, OCN, and RUNX2 messenger RNA expression was examined in osteogenesis-induced calvarial bone osteoblasts treated with or without APR at 80 µM (n = 3). (D) Western blot analysis of OCN protein levels normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The numbers below represented the mean of protein/GAPDH ratios normalized to 1.0 (OM-APR-group) quantified by densitometry. Data are shown as mean ± SD. *P < 0.05. **P < 0.01. ***P < 0.001.