Fig. 2.

Temporal expressions and cellular localization of endogenous brain RXR and SIRT6 after SAH. a Representative western blot bands and quantitative analyses of RXR time course in the left hemisphere after SAH. b Representative western blot bands and quantitative analyses of SIRT6 time course in the left hemisphere after SAH. *P < 0.05 vs sham group. Error bars were represented as mean ± SD. n = 6 per group. c Double immunofluorescence staining for RXR (green) in the neuron (NeuN, red), astrocytes (GFAP, red), and microglia (Iba-1, red) in the left basal cortex at 24 h after SAH. d Double immunofluorescence staining for SIRT6 (red) in the neuron (NeuN, green), astrocytes (GFAP, green), and microglia (Iba-1, green) in the left basal cortex at 24 h after SAH. n = 2 per group. Scale bar, 50 μm. DAPI indicates 4′,6-diamidino2-phenylindole; GFAP glial fibrillary acidic protein, Iba-1 ionized calcium binding adaptor molecule-1, NeuN neuronal nuclear