Fig. 1.

Resuscitated HeLa cells derived CSCs show stemness phenotypic characteristics. The graph shows the SFE of 1^st to 5^th- passaged HeLa cells derived CSCs and parental HeLa cells (a). Western blot analysis of ALDH1, Sox2, CD49f, Nanog, and Oct4 in 1^st to 5^th-passage HeLa cells derived CSCs and parental HeLa cells (b). Immunofluorescence staining of ALDH1, Sox2, CD49f, Nanog, and Oct4 in 5^th-passage HeLa cells derived CSCs and parental HeLa cells, respectively; the white arrows point to positive cells (c). Injection of different density of 5^th-passage HeLa cells derived CSCs and parental HeLa cells generated xenografts in nude mice (d). Western blot analysis of ALDH1, Sox2, CD49f, Nanog, and Oct4 in tumor tissues derived from 5^th-passage HeLa cells derived CSCs or HeLa cells bearing mice (e). Transwell assay showing the migrated cells of 5^th-passage HeLa cells derived CSCs and parental HeLa cells; the histogram shows the number of migrated cells; original magnification, × 400 (f). Western blot analysis of E-cadherin, Vimentin, and N-cadherin in 5^th-passage HeLa cells derived CSCs and parental HeLa cells (g). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars represent 50 μM or 10 μM in inset. Results are shown as mean values ± SD of independent experiments performed in triplicate