Fig. 1.

Zfp644 is expressed in mouse embryonic and adult eye. a Expression of Zfp644 gene in embryonic eye at E9.5,; scale bar, 500 µm. (A’) Focus on eye b Negative control; E9.5; scale bar, 500 µm. (B’) Focus on eye c Expression of Zfp644 gene in eye of E 12.5; scale bar, 50 µm. d Expression of Zfp644 gene in eye of E 14.5; scale bar, 50 µm. e Expression of Zfp644 in adult eye; scale bar, 500 µm. e’ Expression of Zfp644 in adult lens; scale bar, 100 µm. e” Expression of Zfp644 in adult retina scale bar, 100 µm. f Negative control; adult eye; g Protein sequence alignment of human ZNF644 and mouse Zfp644 showing position of the single point mutation at the amino acid at position 673 (asterisk); h Expression of Zfp644 gene in eyes of WT animals