Fig. 5.

Knockdown of LPCAT1 attenuated the PI3K/AKT signaling pathway at least in part by targeting MYC. a GSEA analysis showed that LPCAT1 amplification status was correlated with MYC-activated target geneset and negatively correlated with MYC-suppressed target gene set. b The expression of indicated molecules was determined by Western blotting. c Mice were sacrificed after 4 weeks and tumor lesions were harvested. The protein lysates extracted from the tumors were used for Western blotting to detect the expression of the indicated molecules. d HCC827 and PC-9 cells were incubated with IGF-1 (2 μg/ml) for 24 h and the cell lysates were collected. The expression of indicated molecules was determined by Western blot analysis. e After incubation with IGF-1 (2 μg/ml) for 24 h, the cell activity was detected by CCK-8 assay. f Co-IP and Western blotting indicated the endogenous interaction between LPCAT1 and MYC protein in HCC827 cells. Data shown were the average of three independent experiments with similar results. The data are presented as the mean ± SD, *P < 0.05 as determined by the t-test