Table 1.
UV-melting experiments (Tm/°C).
| ||
|---|---|---|
| RNA Triplex Model | ||
| AG | Tm3(°C) pH 6.0 | Tm2(°C) pH 6.0 |
| none | 41.9 | 60.1 |
| 1 (5 eq) | 51.2 (+9.3) | 68.0 (+7.9) |
| 23 (5 eq) | 53.2 (+11.3) | 66.7 (+6.6) |
| 24 (5 eq) | 52.5 (+10.6) | 67.7 (+7.6) |
| 25 (5 eq) | 51.4 (+9.5) | 68.0 (+7.9) |
| AG | Tm3(°C) pH 7.0 | Tm2(°C) pH 7.0 |
| none | 22.6 | 60.1 |
| 1 (5 eq) | 29.4 (+6.9) | 62.0 (+1.9) |
| 23 (5 eq) | 30.4 (+7.8) | 61.2 (+1.1) |
| 24 (5 eq) | 30.4 (+7.8) | 61.6 (+1.5) |
| 25 (5 eq) | 29.4 (+6.9) | 61.9 (+1.8) |
| DNA Triplex Model | ||
| AG | Tm3(°C) pH 6.0 | Tm2(°C) pH 6.0 |
| none | 25.5 | 54.6 |
| 1 (5 eq) | 40.1 (+14.6) | 56.3 (+1.7) |
| 23 (5 eq) | 38.1 (+12.6) | 55.5 (+0.9) |
| 24 (5 eq) | 36.6 (+11.1) | 56.3 (+1.7) |
| 25 (5 eq) | 38.6 (+13.1) | 55.3 (+0.7) |
| 1 (10 eq) | 44.1 (+18.6) | 56.3 (+1.7) |
| 23 (10 eq) | 41.3 (+15.8) | 55.9 (+1.3) |
| 24 (10 eq) | 39.4 (+13.9) | 56.9 (+2.3) |
| 25 (10 eq) | 42.9 (+17.4) | 56.5 (+1.9) |
ΔTm-values in parentheses. Error limits for each Tm-value (an average of three temperature ramps) were less than 1 °C. Tm3 and Tm2 correspond to melting values of the triplex and duplex, respectively.