Table 1. Properties of probe–hpDNA complexes.
| Probe name | λ abs max (nm) | λ em max (nm) | Brightness a (M–1 cm–1) | Complex stability |
|
| K d1 (nM) | K d2 (nM) | ||||
| Hoechst 33342 | 352 | 455 | 32 460 ± 5480 | 2.6 ± 0.4 | — |
| 5-TMR-Hoechst | 558 | 586 | 11 300 ± 1700 | 365 ± 12 | — |
| 6-TMR-Hoechst | 562 | 585 | 3210 ± 240 | 14 ± 1 | 168 ± 23 |
| 5-580CP-Hoechst | 588 | 613 | 31 330 ± 1800 | 155 ± 14 | — |
| 6-580CP-Hoechst | 594 | 619 | 9600 ± 830 | 27 ± 3 | 362 ± 37 |
| 5-610CP-Hoechst | 614 | 641 | 36 400 ± 3230 | 347 ± 22 | — |
| 6-610CP-Hoechst | 618 | 644 | 22 660 ± 1180 | 65 ± 7 | 4408 ± 1810 |
| 5-GeR-Hoechst | 641 | 660 | 10 440 ± 1340 | 4374 ± 192 | — |
| 6-GeR-Hoechst | 643 | 662 | 10 860 ± 850 | 572 ± 26 | — |
| 5-SiR-Hoechst | 651 | 672 | 14 210 ± 1490 | 4799 ± 201 | — |
| 6-SiR-Hoechst | 654 | 677 | 8500 ± 1690 | 669 ± 26 | — |
aCalculated using the equation: ε × QY; in PBS + hpDNA. Data presented as the mean value with standard deviation, N = 3.