Table 3.

RNA Sequencing Validation Through qPCR.

Genes	RNA Sequencing Data		qPCR Validation
Gene Symbol	Log2 Fold-Change	P-value	Log2 Fold-Change ± SEM	P-value	*P < 0.05 **P < 0.01
Mild IOP
Tsacc	∞	2.20E-03	5.7 ± 1.6	8.38E-02
Cp	2.1	5.05E-03	1.1 ± 0.3	3.01E-02	*
Trf	1.1	2.60E-03	0.7 ± 0.2	3.97E-02	*
Crygs	−∞	5.00E-05	−8.7 ± 1.9	1.18E-02	*
Cryba1	−∞	5.00E-05	−9.2 ± 1.0	1.20E-02	*
Cryaa/Cryaa2	−7.3	5.00E-05	−7.2 ± 1.8	2.89E-02	*
Moderate IOP
Cp	2.5	3.00E-03	2.1 ± 0.6	7.40E-03	**
Trf	2.4	5.00E-05	1.4 ± 0.3	1.60E-03	**
Cryaa/Cryaa2	−∞	5.00E-05	−8.6 ± 3.6	5.81E-02
Cryba1	−∞	5.00E-05	−10.1 ± 1.2	2.78E-02	*
Nab2	−1.9	5.00E-05	−0.9 ± 0.2	3.90E-03	**