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. 2019 Feb 22;9:2555. doi: 10.1038/s41598-019-38805-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Micro MRR system consists of a portable permanent magnet which provides strong magnetic field. A home build radiofrequency (RF) detection probe is connected to RF spectrometer. This probe transmits and receives RF signal from the spectrometer for MRR analysis of the sample inside the RF detection micro coil. The sample, iRBCs/uRBCs pellet was separated from plasma of blood sample to 4 mm detection range of detection coil by using a micro centrifuge (methods)¹. (b) Sample preparation of unlysed uRBCs/iRBCs to micro capillary tube for MRR measurements. Red colour in 4 mm range is the uRBCs/iRBCs pellet separated from PBS (blue colour) by micro centrifuge (methods). (c) Saponin lysis of iRBCs (methods)²³. Sample preparation of lysed uRBCs/iRBCs (d) spinning method: spin down all the pellet (green colour) in to a micro capillary tube and (e) suspension method: dilute the pellet in 20 µl of PBS and fill the 4 mm detection range (brown colour) of micro capillary tube.