Scheme 3. Preparation of the decaborate-albumin 8 and trastuzumab 9via the one-pot three-component double-click labeling method. Purification was performed by ultrafiltration using an Amicon filter, 50 000 molecular weight. Mass analysis of the decaborate-HSA 8 by MALDI-TOF. Dissociation constants (Kd) of the decaborate-trastuzumab 9 measured by the QCM method. DMSO = dimethylsulfoxide. QCM = quartz-crystal microbalance.
