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. Author manuscript; available in PMC: 2019 Feb 22.
Published in final edited form as: Eur J Immunol. 2015 Dec 17;46(3):647–655. doi: 10.1002/eji.201545911

Figure 1.

Figure 1.

Influence of silencing IRFs on CpG-mediated gene activation. CAL-1 cells were transfected with 0.5 nM of each indicated siRNA to silence gene expression. The siRNA transfected cells were stimulated 20 h later with 1 μM of “K” class CpG ODN and IFN-ß mRNA expression assessed by RT-PCR. GAPDH was used as an endogenous control and fold changes in mRNA level determined by comparison to identically treated cells transfected with control siRNA. Data are shown as the mean + SD from 3 independent experiments, each performed in triplicate. **p < 0.01; ANOVA one-way analysis of variance.