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. Author manuscript; available in PMC: 2019 Feb 22.
Published in final edited form as: Cancer Immunol Res. 2016 Dec 5;5(1):61–71. doi: 10.1158/2326-6066.CIR-16-0113

Figure 2.

Figure 2.

IL4-producing cells in the draining lymph nodes (LN) had a Tfh phenotype. A, Tumor-draining lymph nodes of 4GET mice were removed on day 7 and FACS sorted on the basis of their expression of CD3ε, CD4, and IL4-eGFP. B–F, Sorted 4GET cells were analyzed for mRNA expression. Naïve CD4 T cells were sorted on the basis of their expression of CD62L+ and CD44−. Th2 cells were generated by stimulating with IL4, anti-IFNγ and anti-CD3 and anti-CD28 in vitro for 4 days. Results represent the mean + SD of results from 5 to 9 independent sorted cell populations. *, P < 0.05 when compared with Th2 cells. **, P < 0.01 when compared with Th2 cells. G, Naïve or tumor-draining lymph nodes cells isolated from 4GET mice were removed 7 days after tumor inoculation. The expression of eGFP and CD4 in CD3+ cells, or eGFP and CXCR5, PD-1, or ICOS in CD4+ cells were analyzed by flow cytometry. Experiments were repeated three times with similar results.