Table 1.

Increased and decreased expression of helper T-cell–related genes in tumor-draining lymph nodes compared with naïve lymph nodes

Name of gene	Symbol	Fold change	SD	P
Increased expressiona
Interleukin 4	Il4	76.54	36.10	0.022267
Suppressor of cytokine signaling 3	Socs3	4.86	1.39	0.008650
Inducible T-cell co-stimulator	Icos	3.29	0.12	0.000004
Tumor necrosis factor superfamily, member 4	Tnfsf4	3.43	0.16	0.000014
Interleukin 6	Il6	3.20	1.52	0.040122
Chemokine (C-C motif) receptor 3	Ccr3	2.92	0.88	0.019473
Suppressor of cytokine signaling 1	Socs1	2.85	0.34	0.000689
Interleukin 2	Il2	2.79	0.54	0.004394
Janus kinase 2	Jak2	2.78	0.67	0.010155
Interleukin 12b	Il12b	2.74	0.50	0.003800
Interleukin 18	Il18	2.43	0.68	0.021357
Chemokine (C-C motif) receptor 5	Ccr5	2.40	0.57	0.013410
Chemokine (C-C motif) receptor 4	Ccr4	2.35	0.33	0.002088
Cytotoxic T-lymphocyte-associated protein 4	Ctla4	2.33	0.65	0.024373
B-cell CLL/lymphoma 6	Bcl6	2.26	0.14	0.000105
Interleukin 2 receptor, alpha	Il2ra	2.00	0.47	0.021042
Decreased expressionb
Inhibin alpha	Inha	0.217	0.03	0.000004
Polycomb group ring finger 2	Pcgf2	0.409	0.11	0.000899
Interleukin 23, alpha subunit p19	Il23a	0.433	0.34	0.046563
Interleukin 17A	Il17a	0.449	0.19	0.00837
Chemokine (C-C motif) ligand 11	Ccl11	0.456	0.09	0.000618

NOTE: A total of 2.5 × 10⁵ CT26 colon cancer cells were injected subcutaneously into the right flank of syngeneic mice. Tumor-draining lymph nodes were removed on day 4 and analyzed gene expressions by RT² Profiler PCR array. Data show the mean fold change + SD from three independent lymph nodes.

^aA total of 16 genes showed increased expression of 2.0-fold or more.

^bA total of 5 genes showed decreased expression of 0.5-fold or less.