Table 1. FP and competition FP binding data of MccB variants binding to the N-formylated 2 substrate.

Variant	K d (μM)	IC50 (μM)	K i (μM)	[Protein] (μM)
Y14A	143.40 ± 35.90	NA	NA	NA
E26A	91.11 ± 4.70	NA	NA	NA
E26D	539.40 ± 358.40	NA	NA	NA
E26Q	16.93 ± 3.11	59.34 ± 8.75	13.46	30
S212A	2.06 ± 0.05	6.99 ± 1.02	2.87	2
F217A	8.85 ± 0.88	32.07 ± 7.36	11.86	10
I243A	>800	NA	NA	NA
V264A	79.17 ± 12.81	682.50 ± 61.55	399.29	50
Y268A	17.57 ± 1.80	93.35 ± 15.65	37.28	20
Y268F	4.15 ± 0.37	36.83 ± 3.18	15.14	5
H333A	5.01 ± 0.37	26.55 ± 2.98	11.62	5