Table 1. Pyrosequencing of 16S rRNA and alpha-diversity indices.
| Treatment* | High-quality sequences | OTU number | Chao index | Shannon index | SRA database accession no. |
|---|---|---|---|---|---|
| FR-2d | 56,214.0 ± 6,924.0b | 2,066.0 ± 155.0b | 2,068.2 ± 154.7b | 8.3 ± 0.1b | SRP134256 |
| RT-2d | 58,342.0 ± 399.0ab | 1,347.0 ± 63.0c | 1,350.1 ± 63.5c | 7.0 ± 0.1d | |
| GTC-2d | 60,940.0 ± 863.0ab | 2,314.0 ± 37.0a | 2,316.0 ± 37.1a | 8.5 ± 0.0a | |
| FR-8d (RT) | 58,259.0 ± 4,254.0ab | 2,219.0 ± 44.0ab | 2,221.0 ± 44.0ab | 8.5 ± 0.1ab | |
| RT-8d | 62,145.0 ± 646.0a | 1,446.0 ± 63.0c | 1,450.3 ± 62.7c | 7.2 ± 0.2c | |
| GTC-8d | 60,640.0 ± 273.0ab | 2,236.0 ± 48.0ab | 2,237.4 ± 48.4ab | 8.4 ± 0.1ab | |
| FR-31d (RT) | 60,987.0 ± 1,351.0ab | 2,176.0 ± 18.0b | 2,178.0 ± 17.9b | 8.5 ± 0.1ab | |
| RT-31d | 62,335.0 ± 322.0a | 1,192.0 ± 68.0d | 1,195.9 ± 67.6d | 6.9 ± 0.1d | |
| GTC-31d | 61,742.0 ± 1,489.0a | 2,302.0 ± 24.0a | 2,303.6 ± 24.4a | 8.5 ± 0.0a |
Note:
*
Three independent biological replicates were analyzed in each group. Different lower-case letters indicate significant differences between groups analyzed by one-way ANOVA (LSD) analysis (p < 0.05).