Figure 4.

Western blot analysis for proteins relating to ICC, enteric nerves, and MYH11 cells in antrums from Lep^ob and wild-type animals. A: Representative Western blots comparing the expression of various proteins associated with ICC (KIT; DLG4), enteric nerves (NOS1; UCHL1, SNAP25, and syntaxin), and MYH11 in wild-type and Lep^ob mice, respectively. Each band is demarcated by a boundary box to illustrate that they were from different gels or different parts of the same gel. Bands are the most representative from the gastric antrums of three different animals. GAPDH was used as a control housekeeping protein to which the other proteins were compared. Three separate GAPDH blots, one from each animal, are shown. B: Western blot analysis bands from wild-type and Lep^ob antrums were quantified by densitometry and expressed relative to GAPDH. The summary graph demonstrates that the major proteins associated with ICC, enteric neurons, and smooth muscle were unchanged in the antrums of Lep^ob compared with wild-type animals (unpaired Student t test). M, marker.