Skip to main content
. Author manuscript; available in PMC: 2019 Jul 14.
Published in final edited form as: Nat Neurosci. 2019 Jan 14;22(2):218–228. doi: 10.1038/s41593-018-0305-z

Figure 5: Identified LC-GABA units display two types of activity with respect to pupil size.

Figure 5:

a. Examples of simultaneous recordings of pupil size and photo-tagged Dbh- (upper panel) or Gad2-ChR2 (middle and lower panels) LC units. Note the Gad2 unit in the middle panel is positively correlated (Gad2+) while that in the lower panel is negatively correlated with pupil size (Gad2-). b. Cumulative probability distribution of the Pearson correlation coefficient of LC unit spike rate with pupil size for all units. The gray area marks non-significant correlations, p < 0.05. n = 19, 18 and 396 Dbh-ChR2, Gad2-ChR2 and non-identified units from 13 mice. c. Percentage of Dbh- and Gad2-ChR2 units that are positively or negatively correlated with pupil size. Proportions were significantly different for the two groups (19/19 versus 11/18 in the Dbh and Gad2 groups; χ2: 9.11; p = 0.0025). The gray portion indicates non-significant correlations, p < 0.05. d. Average spike rate for different type of units during periods of constricted versus dilated pupil. Each line represents a single unit. Two-tailed paired t-test: ***p = 0.0005 (t18 = 5.36) for Dbh; **p = 0.0014 (t10 = 4.38) for Gad2+; *p = 0.0208 (t5 = −3.33) for Gad2-. e, f. Normalized cross-correlation and delay of pupil size to LC firing activity for the classes of units sorted in (b, c). One-way ANOVA, F2,33 = 6.33, **p = 0.0088 using Tukey post-hoc test. Box plots indicate the median (center line), first quartiles (box edges), minimum/maximum values (whiskers) and outliers (+). Error bars in (d, e) indicates s.e.m. In (d-f) n = 19, 11, and 6 Dbh, Gad2+ and Gad2- units taken from 13 mice.