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. 2018 Dec 4;9(2):345–357. doi: 10.1534/g3.118.200705

Figure 7.

Figure 7

Hipp1 mutant females are fertile and do not phenocopy Su(Hw) loss. A. Fecundity (eggs laid per female per day) of five-day-old Hipp1−/−, su(Hw)−/− and heterozygous Hipp1-/+, su(Hw)-/+ mutant females of the indicated genotypes, crossed to wild type males. The two wild type (+/+) reference strains were Canton S (1) and yw (2). Genotypes are noted under the graph. Error bars indicate the standard deviation from a minimum of three independent experiments. Fecundity was compared between genotypes using a one-way ANOVA followed by Tukey post hoc analysis. Asterisks indicate genotypes that were significantly different from control lines (p value < 0.01). Only su(Hw) null backgrounds showed a significant difference in egg laying. B. Heat map of fold changes of gene expression defined by quantitative reverse transcription PCR (RT-qPCR) of Su(Hw) target genes, measuring gene expression levels in RNA isolated from 1< day-old su(Hw)+/+ (Canton S), two su(Hw)−/−, two Hipp1−/− and one Hipp1−/−, su(Hw) −/− double mutant backgrounds. Fold change in expression was determined by normalizing levels to the housekeeping gene RpL32 and is relative to RNA levels in one of the three su(Hw)+/+ (Canton S) RNA samples. The color key corresponding to fold change is shown below. Asterisks indicate gene expression changes relative to Canton S, * P < 0.05. ** P < 0.01, *** P < 0.001 (Student’s t-test).