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. 2018 Dec 17;9(2):523–533. doi: 10.1534/g3.118.200947

Figure 6.

Figure 6

Detection of interactions between oar-miR-379-5p and WNT8A by dual luciferase reporter system. a. Binding site sequence of miRNA and target gene WNT8A. b. Structure of pmirGLO plasmid vector. c. Working principle of pmirGLO plasmid vector. The dual luciferase reporter gene was inserted the 3′UTR sequence of the target gene, which can bind with miRNA into the downstream of the luciferase gene in pmirGLO plasmid vector, the translation of luciferase from firefly will be inhibited and the luciferase activity will eventually decrease when endogenous miRNA or introduced exogenous miRNA are combined with the inserted 3′UTR sequence. d. Relative luciferase activity after transfecting oar-mir-3799-5p and WNT8A recombinant plasmid for 24h. e. Relative luciferase activity after transfecting oar-mir-3799-5p and WNT8A recombinant plasmid for 48h.