Table 1. qRT-PCR primer information.
Name | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
---|---|---|
hsa-miR-3529-5p | ACGAGGTAGACTGGGATTTGTTGT T | Universal reverse* |
hsa-miR-6820-3p | TGTGACTTCTCCCCTGCCACAG | Universal reverse* |
hsa-miR-3615 | CTCTCGGCTCCTCGCGGC | Universal reverse* |
mmu-miR-1957b | CGCGTGGAATGTAACTCAGTGGTA T | Universal reverse* |
mmu-miR-182-3p | CGGTGGTTCTA ACTTGCCAA CT | Universal reverse* |
mmu-miR-1929-3p | CAGCTCATGGAGACCTAGGTGG | Universal reverse* |
oar-miR-379-5p | CTGGTAGACTATGGAACGTAGGC | Universal reverse* |
oar-miR-665-3p | CCAGTAGGCCGAGGCCCC | Universal reverse* |
oar-miR-200b | ACGCTAATACTGCCTGGTAATGATG | Universal reverse* |
oar-miR-409-5p | CGAATGTTGCTCGGTGAACCCC | Universal reverse* |
oar-miR-495-3p | AGCGAAACAAACATGGTGCACTTCTT | Universal reverse* |
oar-miR-200c | CGTAATACTGCCGGGTAATGATGG | Universal reverse* |
U6 | CGAGGATGTGAAGACACCAAGAC | Universal reverse* |
Universal reverse was provided by manufacture (miRcute Plus miRNA qPCR Detection Kit, TIANGEN BIOTECH, China).