Table 2. Sequence information of wild- and mutant-type target gene fragments and positive control fragments.
Gene name | Sequence information (5′-3′) |
---|---|
WNT8A-WT | Sense: CGGAAGTTGGCATCTCAAGAAAAACCATAAGCAGGTTCTTTGCAAGTCTACCCTTATCTCTGTTTTGC |
Antisense: TCGAGCAAAACAGAGATAAGGGTAGACTTGCAAAGAACCTGCTTATGGTTTTTCTTGAGATGCCAACTTCCGAGCT | |
WNT8A-MUT | Sense: CGGAAGTTGGCATCTCAAGAAAAACCATAAGCAGGTTGTAAGCATCAGATGGCTTATCTCTGTTTTGC |
Antisense: TCGAGCAAAACAGAGATAAGCCATCTGATGCTTACAACCTGCTTATGGTTTTTCTTGAGATGCCAACTTCCGAGCT | |
PC (oar-miR-379-5p inhibitor) | Sense: CGCCTACGTTCCATAGTCTACCAACCGGTGCCTACGTTCCATAGTCTACCAC |
Antisense: TCGAGTGGTAGACTATGGAACGTAGGCACCGGTTGGTAGACTATGGAACGTAGGCGAGCT |