Figure 2.
SNZ1 is required for PLP synthesis in the presence of thiamine. Strains with deletions of SNZ1, SNZ2, SNZ1,2, SNZ1,3, SNZ2,3 and SNZ1,2,3 were grown overnight in YPD and 5 µL each of dilutions from 10−2 to 10−7 were spotted on synthetic dextrose (A) or glycerol (B) media or a dropout derivative of this medium lacking pyridoxine and/or thiamine as indicated. Plates were incubated for two (SD) or three (SG) days at 30 °C. Growth of the strain deleted for SNZ3 was indistinguishable from the snz2 mutant.