Figure 6.

SNZ3 and SNO3 can synthesize PLP in Salmonella enterica. Growth of pdxJ mutant of S. enterica with various plasmids was monitored for growth. In panel (A) the pdxJ mutant carried an empty vector (pSU18) (circles) or a plasmid expressing SNZ3 (squares). Each of these two strains were grown on NCE (i.e., high ammonia) minimal medium with glycerol. Filled and open symbols represent growth in the absence, while solid symbols represent the presence, of 100 nM pyridoxal. In panel (B) the pdxJ mutant carried a plasmid expressing SNZ3. In addition, the strain had a compatible empty vector (triangles), or a plasmid expressing pSNO3 (diamonds). All strains were grown on NCN minimal medium with glycerol and glutamine (i.e., low ammonia). Open symbols represent growth in the absence, while solid symbols represent the presence, of 100 nM pyridoxal. Growth was monitored as a function of optical density at 650 nm with shaking at 37 °C. Error bars indicate the standard deviation of three independent biological replicates.