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. 2018 Dec 27;9(2):591–599. doi: 10.1534/g3.118.200963

Figure 2.

Figure 2

Dox-inducible H2BGFP reporter gene expression in different organs of H11-albumin-rtTA/TetO-H2BGFP double-transgenic mice. (A) Schematic diagram of induced H2BGFP expression in the H11-albumin-rtTA/TetO-H2BGFP mice after Dox treatment. The rtTA protein binds with Dox and forms a complex that initiates transcription of a H2BGFP fusion protein construct positioned downstream of a tetracycline inducible promoter (TetO-CMV). (B) PCR genotyping of mice. The positive transgenic mice had a 1.9 kb product for rtTA; H2BGFP generated a 191 bp product. Lanes 1 and 4 were transgene+ mice; lanes 2, 3, and 5 were transgene- mice. (C) qRT-PCR analysis of the relative expression of H2BGFP mRNA in different organs dissected from double transgenic mice treated with 1mg/ml Dox (+Dox) or water (-Dox) for 48 h (H2BGFP expression was normalized to Gapdh (n = 4)). Error bars show mean ± SEM (n = 4). (D) Light images (top panels) and whole mount fluorescence microscopy (bottom panels) analyses of H2BGFP protein in the Dox treated H11-albumin-rtTA/TetO-H2BGFP mice. Scale bar: 5 mm.