Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 21;9(2):491–503. doi: 10.1534/g3.118.200874

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Matera et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Flag-SMN immunopurified lysates contain members of the known SMN complex and known substrates. (A) Flag-purified eluates were analyzed by label-free mass spectrometry. Proteins in the core SMN complex and known substrates were highly enriched in the SMN sample as compared with Ctrl. (B) Lysates from Oregon-R control (Ctrl) Drosophila embryos and those that exclusively express Flag-SMN (SMN) were immunopurified. Protein eluates were separated by SDS-PAGE and silver stained. Band identities were predicted by size. (C) Following transfection of CG2941-Flag in Drosophila S2 cells and immunoprecipitation (IP) of total cell lysates with anti-Flag beads, western analysis was carried out using anti-SMN antibodies. Mock transfected cells were analyzed in parallel. Co-purification of endogenous SMN was detected in the CG2941-Flag IP lane but not in the control lane (Mock).