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. 2018 Dec 21;9(2):491–503. doi: 10.1534/g3.118.200874

Figure 7.

Figure 7

CG2941 and its paralogs are essential for viability and locomotion independent of snRNP biogenesis. (A) daughterless (Da) or tubulin (Tub) Gal4 drivers were crossed to RNAi lines specifically targeting CG2941 (HMJ-21393) or all three paralogs (HMJ-22884). 100 larvae per cross were selected and viability was measured based on the number of larvae that pupated (darker bars) and that eclosed (lighter bars). “n/a” represents a category in which there were no pupae (pupation) or adults (eclosion) to count. Error bars show standard error of the mean. (B) Wandering third instar larvae from the same crosses in (A) were recorded and locomotor behavior was measured using the wrMTrck plug-in for Fiji/ImageJ. Error bars represent standard error. (C) Early third instar larvae (73-77 hr post-egg laying) from the same crosses in (A) were collected and RNA was extracted to measure snRNA levels via northern blotting. See Fig. S2 for details on the knockdown. Probe sequences are described in Materials and Methods.